Journal: Biochimica et biophysica acta. Molecular basis of disease

Article Title: Genome-wide translational reprogramming of genes important for myocyte functions in overload-induced heart failure.

doi: 10.1016/j.bbadis.2019.165649

Figure Lengend Snippet: Fig. 1. Time-dependent changes in activation of the mTOR and eIF2α pathways, and changes in ribo- somal protein levels in pressure overload-treated left ventricles (LVs). (A) Western blots and densitometry data showing the phosphorylation levels of mTOR, 4EBP and eIF2α in sham and transverse aortic con- striction (TAC)-treated LV tissues. (B) Western blots and densitometry data showing changes of ribosomal proteins RpS6, RpS7 and RpL10A in TAC-treated LV tissues. Data are mean ± SEM. ⁎P < .05 vs sham, †P < .05 vs TAC-2W, one-way ANOVA followed by Tukey's test (n = 3–4).

Article Snippet: A cDNA clone of constitutively active human eIF2α (eIF2α-S51A) was obtained from Addgene (Cambridge, MA, USA) (plasmid #21808, a gift from Professor David Ron, University of Cambridge).

Techniques: Activation Assay, Western Blot, Phospho-proteomics

Journal: Biochimica et biophysica acta. Molecular basis of disease

Article Title: Genome-wide translational reprogramming of genes important for myocyte functions in overload-induced heart failure.

doi: 10.1016/j.bbadis.2019.165649

Figure Lengend Snippet: Fig. 5. Cardiac-specific overexpression of eIF2α-S51A improved the translational efficiency of cardiac genes and ameliorated cardiac dysfunctions at 5 weeks after TAC. (A) Changes in cardiac contractility (EF and FS), chamber size (LVIDd) and wall thickness (IVSd) after cardiac overexpression of eIF2α-S51A. GFP over- expression was used as control. (B) Alterations of the relative translational efficiency of selected cardiac genes (assays performed with purified myocytes). (C) Immunohistochemical staining for VEGF-A (brown color) in GFP- and eIF2α-S51A-transduced myocardium. Quantitative immunoreactivity data were shown in the bar graph. The protein translational efficiency was estimated by normalizing the immunoreactivity data with corresponding mRNA values. Scale bar = 20 μm. (D) Microvessels detected by immunohistochemical staining for von Willebrand factor (brown color) in GFP- and eIF2α-S51A-transduced myocardium. The average vessel density was shown in the bar graph. Scale bar = 100 μm. Data were mean ± SEM (n = 10 for A; n = 4–5 for B to D). ⁎P < .05 versus GFP (control), one-way ANOVA or unpaired t-test.

Article Snippet: A cDNA clone of constitutively active human eIF2α (eIF2α-S51A) was obtained from Addgene (Cambridge, MA, USA) (plasmid #21808, a gift from Professor David Ron, University of Cambridge).

Techniques: Over Expression, Control, Immunohistochemical staining, Staining